Nanocin-plasmid™

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plasmid transfection reagent

Finally , transfection of a wide range of cell types can now be achieved easily and efficiently and without cell toxicity.

The scientists at Tecrea Ltd have developed Nanocin-plasmid™ technology to overcome major limitations with existing transfection procedures, which often suffer from difficult protocols, poor efficiency and unacceptable levels of toxicity to cultured cells.

Nanocin-plasmid™ is effectively non-toxic to precious cultured cells, including primary cells, which will greatly improve the scientific integrity of your experiments. Nanocin-plasmid™ is a newly developed product, but already it has found success in a range of applications and cell types. A key feature of Nanocin-plasmid™ is that it efficiently enters the cytoplasm of mammalian cells. When mixed with DNA, Nanocin-plasmid™ forms nanoparticles that enable efficient plasmid transfection without toxicity. This may sound complicated, but our products and protocols make it east. Being effectively non-toxic, Nanocin-plasmid™ can be used for repeated transfections.

Nanocin-plasmid™ provides a flexible new system for plasmid transfection.  Importantly, the underlying chemistry involved is compatible with clinical development.

Technology
Combining Nanocin-plasmid™ with plasmid results in rapid self assembly of nanoparticles. These particles massively enhance plasmid transfection into a wide range of cell types. Moreover, as the platform is so safe there is no need to wash the cells following transfection. Indeed, we have shown that repeated transfection is feasible with no detrimental impact on the cells. Nanocin-plasmid™ can be used with even the most sensitive primary cells and the chemistry is compatible with in vivo applications.

Cell Lines
Nanocin-plasmid™ is suitable for plasmid transfection in a wide range of cell lines.  Feel free to contact us if you have specific questions.

  • HeLa
  • HEK293
  • SAOS2
  • Neuro2a
  • RPE-1
  • HaCat
  • Neb1s
  • CHO
  • 1Adipocytes
  • 293T
  •  HUVEC
  • MDBK
  • 3T3
  • MCF7
  • Granulosa cells
    (somatic cells from ovary folicles)
  • Granulosa cell line
  • mature Leukaemia T cell
  •  Sf9 Cells 
  • Human RPE1, (retinal pigment epithelial cells)

Rapid Protocols
The very low toxicity nature of Nanocin-plasmid™ enables a rapid protocol to transfect plasmid DNA into mammalian cells at the time of splitting or seeding. The rapid protocol almost halves the time of a standard protocol and reduces the protocol by several steps. Full protocols can be found on our website.

If you are interested in siRNA transfection reagents, see:

To purchase directly from Tecrea, please request a quote or submit a purchase order via [email protected]

Alternatively, purchase through one of our distributors.