No, all of our Nanocin protocols involve only standard lab equipment. Conveniently, there is no need to purchase new equipment or specialised disposables.

Nanocin products are effectively non-toxic and are particularly recommended for transfection of highly sensitive cells, as they do not compromise cell viability.

Of course, all substances are toxic at some level, but we are confident that Nanocin products are of a significantly lower level of toxicity compared to other transfection technologies.

We take pride in working to ensure that Nanocin is gentle to cells in vitro and in vivo. Please contact us to discuss any queries you may have concerning toxicity data for Nanocin products.

Yes, each cell line may require optimisation of transfection conditions. The Nanocin protocols, available in each reagent’s page, provide a convenient starting point. At Tecrea we are constantly working to provide optimum protocols for specific cell lines e.g. CHO, HEK, HeLa, and many more. You can contact us for advice and suggestions.

Yes, cell density will affect transfection efficiencies and reagent requirements. Please check the protocols for density recommendations.

Nanocin products work well in the presence or absence of antibiotics properties.

Yes, Nanocin products work in the presence of serum. However, as with most or all transfection reagents, Nanocin is more efficient when used in the absence of serum. For optimal results, particularly when working with difficult to transfect cells, we suggest transfection in the absence of serum.

The complexation time is not critical, but we recommend using a consistent incubation period when conducting different experiments.

Yes. Nanocin products are effectively non-toxic, therefore it is possible to perform multiple consecutive transfections on cells in order to obtain a prolonged effect of overexpression or knockdown.

Nanocin products can be used to transfect DNA, RNA, siRNA, antisense DNA, PNA, oligonucleotides, small molecules, and proteins including antibodies and peptides.

Yes, Nanocin products have been validated to effectively and reproducibly co-transfect DNA plasmids to a variety of mammalian cells.

Yes, however it is important to mix plasmid with Nanocin PLASMID reagent and siRNA with Nanocin RNA reagent and add to cells separately.

Nanocin products are very stable and can be shipped at room temperature. They can be stored at 2-8°C for at least two years, or stored at room temperature on the bench for up to one month. Wherever they are stored, Nanocin products should be protected from light to retain stability.

“Nanotechnology is the term given to those areas of science and engineering where phenomena that take place at dimensions in the nanometre scale are utilised in the design, characterisation, production and application of materials, structures, devices and systems.”

– Scientific Commitee on Emerging and Newly Identified Health Risks of the European Comission.

To learn more about nanoparticles and nanotechnology, please follow this link.

Nanocin is a very stable reagent. At the time of dispatch, we ensure that the expiry date on kits is at least one year. We welcome any questions about the stability or shelf life of the reagents.

Yes, but each cell line may require optimisation of transfection conditions. The Nanocin protocols provide a convenient starting point and or you can contact us for further guidance.

Yes. Nanocin PLASMID will complex with all types of nucleic acids. Plasmid are the most common type of DNA used in delivery, but the protocols can be applied to any form of DNA as long as you know the concentration.

Yes; the buffer is optimised to improve efficiency and consistency.

Yes, but each cell line may require optimisation of transfection conditions. The Nanocin protocols provide a convenient starting point and or you can contact us for further guidance.

Yes, but each cell line may require optimisation of delivery conditions.

Formally, transfection is used in association with nucleic acids, but it is often used loosely to describe the delivery of other types of compounds into cells. At Tecrea, we prefer to say that Nanocin PRO is a “delivery reagent”.

Yes, but each cell line may require optimisation of delivery conditions.

Formally, transfection is used in association with nucleic acids, but it is often used loosely to describe the delivery of other types of compounds into cells. At Tecrea, we prefer to say that Nanocin SM is a “delivery reagent”.